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Inserts encoding the neoAgs were obtained from Genscript and cloned into MSCV-IRES-Thy1.1 DEST and pBK-T-SFV-b12A utilizing BglII&SalI and Apa I websites, respectively. MSCV-IRES-Thy1.1 DEST was a gift from Anjana Rao (Addgene plasmid # 17442). Plasmids pMSCV-TMGP69-IRES-Thy1.1, pMSCV-TMGP73-IRES-Thy1.1 encoding the neoAgs and management plasmid (MSCV-IRES-Thy1.1 DEST) had been used to arrange retrovirus for transduction experiments. A needle tumor biopsy was extracted at analysis and preserved at -80°C in RNAlater.

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